-Tubulin during Acentriolar Plant Mitosis
نویسندگان
چکیده
Jaroslav Dole el, b László Bögre, d and Pavel Dráber e a Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic b Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, 772 00 Olomouc, Czech Republic c Institute of Plant Biochemistry, P.O. Box 110432, D-06018 Halle, Germany d School of Biological Sciences, Royal Holloway, University of London, Surrey, United Kingdom e Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic
منابع مشابه
Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells.
The spindle pole localization of gamma-tubulin was compared in wild type and acentriolar cultured Drosophila cells using polyclonal antibodies specifically raised against the carboxy terminal amino acid sequence of Drosophila gamma-tubulin-1 (-KSEDSRSVTSAGS). During interphase, gamma-tubulin was present in the centrosome of wild type cells and accumulated around this organelle in a cell cycle d...
متن کاملCharacterization and immunocytochemical distribution of calmodulin in higher plant endosperm cells: localization in the mitotic apparatus
In this study we have examined the immunocytochemical distribution of calmodulin during mitosis of higher plant endosperm cells. Spindle development in these cells occurs without centrioles. Instead, asterlike microtubule converging centers appear to be involved in establishing spindle polarity. By indirect immunofluorescence and immunogold staining methods with anti-calmodulin antibodies, we f...
متن کاملMicrotubule cytoskeleton remodeling by acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells.
Cytoskeleton microtubules undergo a reversible metamorphosis as cells enter and exit mitosis to build a transient mitotic spindle required for chromosome segregation. Centrosomes play a dominant but dispensable role in microtubule (MT) organization throughout the animal cell cycle, supporting the existence of concurrent mechanisms that remain unclear. Here we investigated MT organization at the...
متن کاملgamma-Tubulin is present in acentriolar MTOCs during early mouse development.
gamma-Tubulin, a recently discovered member of the tubulin superfamily, is a peri-centriolar component considered to be essential for microtubule nucleation. Mouse oocytes and early embryos lack centrioles until the blastocyst stage. Thus, early mouse embryos allowed us to study the location of gamma-tubulin in animal cells in the absence of centrioles. For this, we used an antiserum directed a...
متن کاملEstablishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant
In animal cells the centrosome is commonly viewed as the main cellular structure driving microtubule (MT) assembly into the mitotic spindle apparatus. However, additional pathways, such as those mediated by chromatin and augmin, are involved in the establishment of functional spindles. The molecular mechanisms involved in these pathways remain poorly understood, mostly due to limitations inhere...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2000